In this video, you will see the techniques required to run a UV-visible spectrum. The theory side of UV-vis can be found in a presentation elsewhere. This is the setup required for UV-vis: on the right is a spectrometer, and on the left is the computer which will be used to analyze the data. First, fill two cuvettes with ionized water. This will be used for a reference. It is important to only touch two sides of the cuvettes, as the other two sides must be clear for the beam to pass through. So the cuvettes will have dimples on one side. You handle the cuvettes and then place them inside the spectrometer and close the lid. To run the reference, you click "Auto Zero" in the UV-vis program and click "OK". To run your sample, take a small part of your sample, typically the end of a spatula, and place it into a beaker. Dissolve it in the ionized water. Then, using a pipette, transfer your sample into the cuvette, leaving about one centimeter to the top. It's important not to overfill it. Wipe the cuvette down to make sure there are no finger marks, and place the cuvettes into the first hole in the UV-vis spectrometer, still leaving one blank cuvette in the second hole. Now, click "Start" to run the program, and this will acquire your data for you. Try to ensure that the concentration of your solution isn't too strong, so the peak goes above the graph, or too weak, so there is no peak. The example shown here is about perfect. Once the data acquisition is finished, add labels using the program, marking the height of the peak and what the substance is. You can also carry out UV-vis without the need for a computer; in this...
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