Video instructions and help with filling out and completing Are Form 8453 S Pdf

Instructions and Help about Are Form 8453 S Pdf

In this video you will see the techniques required to run a UV visible spectrum the theory side of UV vis can be found in a presentation elsewhere this is the setup required for UV vis on the right is a spectrometer and on the left the computer which will be used to analyze the data first fill two cuvettes with the ionized water this will be used for a reference it is important to only touch two sides of the cuvettes as the other two sides must be clear for the beam to pass through so i'm cuvettes will have dimples on that be this side you handle the cuvettes are then placed inside the spectrometer and lid close to run the reference you click Auto zero in the UV vis program and click OK to run your sample take a small part of your sample typically the end of a spatula place into a beaker and dissolve in the ionized water then using a pipette transfer your sample into the cuvette leaving about one centimeter to the top it's important not to overfill it wipe the cuvette down to make sure there's no finger marks and place the cadets into the first hole in the UV vis spectrometer still leaving one blank cuvette in the second hole now click starts to run the program and this will acquire your data for you try to ensure that the concentration of your solution isn't too strong so the peak goes above the graph or too weak so there is no peak the example shown here is about perfect once the data acquisition is finished add labels using the program marking the height of the peak and what the substance is you can also carry out uv-vis without the need for a computer in this case the data appears on the screen on the top of the spectrometer this is known as single beam acquisition